BioBlitz Report

From a single tree to a BioBlitz

On the 17 and 18 June 2022 naturalists and the public came together at Little Sparta, a garden in the Pentland Hills 25 miles southwest of Edinburgh, to record the garden’s wildlife for the first time. This concerted effort, known as a BioBlitz, involved 15 local naturalists with expertise in plants, fungi and animals. The aim of a BioBlitz is to record as many species as possible in a set period. Public engagement is also a component of many BioBlitz events, and this was helped using the iNaturalist app that enables complete beginners to contribute biological records using their smartphones.

The event was part of the public engagement activity for the Darwin Tree of Life project which aims to generate complete genome sequences for 70,000 British species. One of the biggest challenges with this ambitious target is finding the species in the first place and the role of amateur naturalists in this is going to be critical. As well as showcasing the value of biological recording, the event was a potential source of samples for genome sequencing from the current hitlist of 2,000 species. By the end of the event 21 species, mostly moths and beetles, had been sampled for genome sequencing at the Wellcome Sanger Institute in Cambridge. These samples will hopefully generate the first genome sequences for these species, giving us greater potential to understand how species interact and survive.

One of the fascinating things about Little Sparta is that it can be viewed as an ecological study into the ability of nature to reassert itself in a place after a long period of suppression, although the motivations behind the creation of the garden were somewhat different. The artist Ian Hamilton Finlay and his wife Sue began the process of creating the garden at Little Sparta in 1966. The garden became the setting for over 270 artworks by Finlay in a series of specific landscapes and the entire garden is Finlay’s greatest work of art.

When work began on the garden there was a single tree, an ash, and a farmhouse needing renovation. The land that became a garden was a closely grazed sheep pasture, which from an ecological perspective would have been described as degraded. The natural state of this land would be a mixed broadleaved woodland of upland tree species such as ash, wych elm, birch, oak and willows. Having been denuded of the natural tree cover over millennia, and with the grassland so heavily grazed, the ecological opportunities afforded through shelter, food and varied environmental conditions became extremely limited. Looking beyond the fence of Little Sparta today gives a good sense of how suppressed nature would have been in 1966.

More than 50 years on, the garden is an oasis for wildlife and a testament to the power of gardening to create habitats that plants, fungi and animals can exploit. But this outcome was not inevitable. Some gardening styles and practices are not particularly wildlife friendly. Fortunately for us, and the species of the Pentland Hills, one of Finlay’s interests was the relationship between humans and nature, so the development of a biodiverse garden was in some sense always part of the vision.

Now that the biological records made during the BioBlitz are largely collated, the purpose of this post is to review what was found and give some context to the findings. The final total, excluding some yet to be identified moths and other insects, was 326 species. This total is very respectable when you consider that lichens, a biologically diverse group that are clearly abundant at Little Sparta, were not properly assessed. The breakdown into groups of organisms was as follows: fungi 16 (5%), birds 22 (7%), other animals (excluding moths) 54 (17%), moths 70 (21%), mosses and liverworts 74 (23%) and vascular plants 90 (27%).

A pattern that has emerged in previous BioBlitz events held by the Botanics is that both moths and bryophytes (mosses and liverworts) have turned out to be particularly rich components of the overall biota. Little Sparta is no exception to this, with counts for moths and bryophytes of 70 and 74, respectively. In part this reflects the fact that both groups are rich in species. However, it is interesting to note that both moths, by virtue of flight, and bryophytes, by virtue of microscopic spores, are relatively mobile species that may more readily colonize suitable new habitat as it develops. This could in part explain why these groups are so well represented at Little Sparta. Conversely, a reasonable prediction would seem to be that less mobile species are likely to be underrepresented at Little Sparta. Clearly, Little Sparta deserves further study. Not least because the species that can be recorded will change with the seasons. Moths are a good example of a group that will be represented by different species at different times of year.

From a public engagement perspective, the iNaturalist app opened new opportunities. People who had not done biological recording before were able to contribute records and found the app easy to use. Unknown species can still be recorded, and identifications can be suggested by other users. Often these suggestions came from others at the BioBlitz, but an interesting outcome was the more than 40 other users of the app who were not present at Little Sparta who helped with species identifications. The app did seem to live up to the billing of being a community for naturalists.

At the time of writing, over 75% of the 362 records made via the app had reached what is called ‘research grade’. This is a data quality check that involves a second user having agreed with an existing identification. Such records can then flow into a data checking process that could see them becoming part of the national biological records that are publicly available online via the National Biodiversity Network (NBN).

Some of the naturalists preferred to stick to their standard recording procedures and so efforts were made to capture as many of these paper records as possible with the app. This involved pairing naturalists with someone who was using the app. Some of the naturalists even had their opinions of the app altered over the course of the event. A feature of the app is that it uses image-recognition to suggest identifications. Moths proved to be a group that the app was pretty good at identifying. In one case the moth expert revised their opinion when the app identification was not in agreement. A closer examination proved that the app was right in its suggestion! At the end of play on the Sunday 204 species had been recorded using the app. This represents 62% of the of the final count of 326 species that includes all records provided by 29 June.

Public involvement was restricted by poor weather. On day one there was near constant rain and on day two the wind was persistent and made conditions feel cold. In total, 24 members of the public participated. The bulk of these were adults, although ten children under ten came as part of family groups. In addition. a local primary school brought six pupils accompanied by two teachers and they participated in a guided activity looking at nature with a focus on ponds. Five students from a local college helped with the running of the event and particular thanks go to them and the team of 15 naturalists who were crucial to the success of the recording effort. It is worth noting that most of the naturalists were not professional scientists, highlighting that so-called ‘citizen scientists’ are a key part of both BioBlitz events and general biological recording activity.

A trial was conducted to see whether water samples from the garden’s four ponds could be used to identify microscopic plants and other forms of pondlife from what is called environmental DNA (eDNA). The method involves filtering water samples to trap DNA on specially designed filters. The samples will be processed in the labs of the Marine Biological Association in Plymouth and will potentially add further to the total species list for the site. Microscopic life can be very challenging to identify by looking down a microscope and this approach could provide new ways of assessing the biodiversity of water bodies. The trial was conducted with a view to streamlining the sampling procedure so that citizen scientists could reliably and easily carry out the sampling.

An update on the results of the eDNA work at Little Sparta will be provided when the samples have been analyzed. As DNA assays aid rapid identification through technologies such as DNA barcoding this will revolutionize how we monitor species. The work of the Darwin Tree of Life will be critical in supporting the development of these new technologies through the provision of entire genome sequences.

The team behind the BioBlitz would like to say a big thank you to all who helped and participated. The staff and friends of Little Sparta showed great enthusiasm for the BioBlitz and supported both the planning and delivery of the event. The success of the event reflects this effort.

By Max Coleman

Science Communicator at the Royal Botanic Garden Edinburgh



The BioBlitz is being run in partnership between The Little Sparta Trust, the Royal Botanic Garden Edinburgh and the Marine Biological Association. Financial support from the Wellcome Trust via the Darwin Tree of Life project is gratefully acknowledged.



RBGE Home | Royal Botanic Garden Edinburgh (

MBA Marine Biological Association | (

PDF about the Jennifer Owens study Jennifer Owens studies.pdf (

Summary of what a BioBlitz is on the NHM website BioBlitz | Natural History Museum (

iNaturalistUK homepage A Community for Naturalists · iNaturalist United Kingdom

Android Google Play Android Apps on Google Play

Apple App Store App Store – Apple

National Biodiversity Network National Biodiversity Network (

Darwin Tree of Life Darwin Tree of Life – Reading the genomes of all life: a new platform for understanding our biodiversity